* [gentoo-commits] proj/sci:master commit in: sci-biology/reapr/files/, sci-biology/reapr/
@ 2016-03-31 22:52 99% Martin Mokrejs
0 siblings, 0 replies; 1+ results
From: Martin Mokrejs @ 2016-03-31 22:52 UTC (permalink / raw
To: gentoo-commits
commit: c2774f23f13407f20176e96d3c85d8918c8ca9c1
Author: Martin Mokrejš <mmokrejs <AT> fold <DOT> natur <DOT> cuni <DOT> cz>
AuthorDate: Thu Mar 31 22:50:10 2016 +0000
Commit: Martin Mokrejs <mmokrejs <AT> fold <DOT> natur <DOT> cuni <DOT> cz>
CommitDate: Thu Mar 31 22:50:10 2016 +0000
URL: https://gitweb.gentoo.org/proj/sci.git/commit/?id=c2774f23
sci-biology/reapr: quick and dirty Debian patches do not help me with sci-biology/bamtools-2.3.0 installed site-wide; the bundled bamtools do not start to compile and tehrefore reapr linking steps fail while using using system-wide incompatible version
Package-Manager: portage-2.2.28
sci-biology/reapr/files/fix_sort_samtools13.patch | 16 ++
sci-biology/reapr/files/use_shared_libs.patch | 252 ++++++++++++++++++++++
sci-biology/reapr/reapr-1.0.18.ebuild | 12 +-
3 files changed, 277 insertions(+), 3 deletions(-)
diff --git a/sci-biology/reapr/files/fix_sort_samtools13.patch b/sci-biology/reapr/files/fix_sort_samtools13.patch
new file mode 100644
index 0000000..f009223
--- /dev/null
+++ b/sci-biology/reapr/files/fix_sort_samtools13.patch
@@ -0,0 +1,16 @@
+Description: adapt samtools sort usage to 1.3 syntax
+ Samtools 1.3 changed the syntax of the 'samtools sort' command, breaking
+ REAPR's smaltmap command in the process.
+ Discussed and forwarded to upstream in person, hence no URL.
+Author: Sascha Steinbiss <sascha@steinbiss.name>
+--- a/src/task_smaltmap.pl
++++ b/src/task_smaltmap.pl
+@@ -155,7 +155,7 @@
+ . " | $samtools view -S -T $assembly -b - > $raw_bam";
+
+ # sort the bam by coordinate
+-push @commands, "$samtools sort $raw_bam $raw_bam.sort";
++push @commands, "$samtools sort $raw_bam -O bam -o $raw_bam.sort.bam";
+
+ # remove duplicates
+ push @commands, "$samtools rmdup $raw_bam.sort.bam $rmdup_bam";
diff --git a/sci-biology/reapr/files/use_shared_libs.patch b/sci-biology/reapr/files/use_shared_libs.patch
new file mode 100644
index 0000000..b748c7e
--- /dev/null
+++ b/sci-biology/reapr/files/use_shared_libs.patch
@@ -0,0 +1,252 @@
+Description: Use_shared_libs
+--- a/src/Makefile
++++ b/src/Makefile
+@@ -1,7 +1,6 @@
+-BAMTOOLS_ROOT = $(CURDIR)/bamtools
+ CC = g++
+-CFLAGS = -Wl,-rpath,$(BAMTOOLS_ROOT)/lib -Wall -O3 -I $(BAMTOOLS_ROOT)/include -L $(BAMTOOLS_ROOT)/lib
+-TABIX = tabix/tabix.o -L./tabix -ltabix -lz
++CFLAGS = -Wall -O3 -I /usr/include/bamtools -I /usr/include
++TABIX = -ltabix -lhts -lz
+ STATS_OBJS = trianglePlot.o coveragePlot.o fasta.o histogram.o utils.o
+ SCORE_OBJS = errorWindow.o utils.o histogram.o
+ BREAK_OBJS = fasta.o utils.o
+@@ -35,46 +34,46 @@
+ $(CC) $(CFLAGS) -c histogram.cpp
+
+ utils.o: utils.cpp
+- $(CC) $(CFLAGS) -lbamtools -c utils.cpp
++ $(CC) $(CFLAGS) -c utils.cpp
+
+ task_stats: task_stats.o $(STATS_OBJS)
+- $(CC) $(CFLAGS) task_stats.o $(STATS_OBJS) -lbamtools -o task_stats $(TABIX)
++ $(CC) $(CFLAGS) task_stats.o $(STATS_OBJS) -lbamtools $(TABIX) -o task_stats
+
+ task_stats.o: task_stats.cpp $(STATS_OBJS)
+ $(CC) $(CFLAGS) -c task_stats.cpp
+
+ task_score: task_score.o $(SCORE_OBJS)
+- $(CC) $(CFLAGS) task_score.o $(SCORE_OBJS) -lbamtools -o task_score $(TABIX)
++ $(CC) -o task_score $(CFLAGS) task_score.o $(SCORE_OBJS) -lbamtools $(TABIX)
+
+ task_score.o: task_score.cpp $(SCORE_OBJS)
+ $(CC) $(CFLAGS) -c task_score.cpp
+
+ task_break: task_break.o $(BREAK_OBJS)
+- $(CC) $(CFLAGS) task_break.o $(BREAK_OBJS) -lbamtools -o task_break $(TABIX)
++ $(CC) $(CFLAGS) task_break.o $(BREAK_OBJS) -lbamtools $(TABIX) -o task_break
+
+ task_break.o: task_break.cpp $(BREAK_OBJS)
+ $(CC) $(CFLAGS) -c task_break.cpp
+
+ bam2fragCov: bam2fragCov.o $(BAM2COV_OBJS)
+- $(CC) $(CFLAGS) bam2fragCov.o $(BAM2COV_OBJS) -lbamtools -o bam2fragCov $(TABIX)
++ $(CC) $(CFLAGS) bam2fragCov.o $(BAM2COV_OBJS) $(TABIX) -lbamtools -o bam2fragCov
+
+ bam2fragCov.o: bam2fragCov.cpp $(BAM2COV_OBJS)
+ $(CC) $(CFLAGS) -c bam2fragCov.cpp
+
+ bam2insert: bam2insert.o $(BAM2INSERT_OBJS)
+- $(CC) $(CFLAGS) bam2insert.o $(BAM2INSERT_OBJS) -lbamtools -o bam2insert $(TABIX)
++ $(CC) $(CFLAGS) bam2insert.o $(BAM2INSERT_OBJS) $(TABIX) -lbamtools -o bam2insert
+
+ bam2insert.o: bam2insert.cpp $(BAM2INSERT_OBJS)
+ $(CC) $(CFLAGS) -c bam2insert.cpp
+
+ bam2perfect: bam2perfect.o $(BAM2PERFECT_OBJS)
+- $(CC) $(CFLAGS) bam2perfect.o $(BAM2PERFECT_OBJS) -lbamtools -o bam2perfect $(TABIX)
++ $(CC) $(CFLAGS) bam2perfect.o $(BAM2PERFECT_OBJS) $(TABIX) -lbamtools -o bam2perfect
+
+ bam2perfect.o: bam2perfect.cpp $(BAM2PERFECT_OBJS)
+ $(CC) $(CFLAGS) -c bam2perfect.cpp
+
+ bam2fcdEstimate: bam2fcdEstimate.o $(BAM2FCDESTIMATE_OBJS)
+- $(CC) $(CFLAGS) bam2fcdEstimate.o $(BAM2FCDESTIMATE_OBJS) -lbamtools -o bam2fcdEstimate $(TABIX)
++ $(CC) $(CFLAGS) bam2fcdEstimate.o $(TABIX) $(BAM2FCDESTIMATE_OBJS) -o bam2fcdEstimate
+
+ bam2fcdEstimate.o: bam2fcdEstimate.cpp $(BAM2FCDESTIMATE_OBJS)
+ $(CC) $(CFLAGS) -c bam2fcdEstimate.cpp
+@@ -110,13 +109,13 @@
+ $(CC) $(CFLAGS) -c scaff2contig.cpp
+
+ task_gapresize: task_gapresize.o $(GAPRESIZE_OBJS)
+- $(CC) $(CFLAGS) task_gapresize.o $(GAPRESIZE_OBJS) -lbamtools -o task_gapresize $(TABIX)
++ $(CC) $(CFLAGS) task_gapresize.o $(TABIX) $(GAPRESIZE_OBJS) -o task_gapresize
+
+ task_gapresize.o: task_gapresize.cpp $(GAPRESIZE_OBJS)
+ $(CC) $(CFLAGS) -c task_gapresize.cpp
+
+ task_fcdrate: task_fcdrate.o $(FCDRATE_OBJS)
+- $(CC) $(CFLAGS) task_fcdrate.o $(FCDRATE_OBJS) -lbamtools -o task_fcdrate $(TABIX)
++ $(CC) $(CFLAGS) task_fcdrate.o $(TABIX) $(FCDRATE_OBJS) -o task_fcdrate
+
+ task_fcdrate.o: task_fcdrate.cpp $(FCDRATE_OBJS)
+ $(CC) $(CFLAGS) -c task_fcdrate.cpp
+--- a/src/task_break.cpp
++++ b/src/task_break.cpp
+@@ -10,7 +10,6 @@
+ #include <assert.h>
+ #include "fasta.h"
+ #include "utils.h"
+-#include "tabix/tabix.hpp"
+
+ using namespace std;
+
+--- a/src/task_score.cpp
++++ b/src/task_score.cpp
+@@ -18,7 +18,6 @@
+ #include "histogram.h"
+ #include "api/BamMultiReader.h"
+ #include "api/BamReader.h"
+-#include "tabix/tabix.hpp"
+
+ using namespace BamTools;
+ using namespace std;
+--- a/src/task_stats.cpp
++++ b/src/task_stats.cpp
+@@ -18,7 +18,6 @@
+ #include "utils.h"
+ #include "api/BamMultiReader.h"
+ #include "api/BamReader.h"
+-#include "tabix/tabix.hpp"
+
+ using namespace BamTools;
+ using namespace std;
+--- a/src/utils.h
++++ b/src/utils.h
+@@ -11,7 +11,7 @@
+
+ #include "api/BamMultiReader.h"
+ #include "api/BamReader.h"
+-#include "tabix/tabix.hpp"
++#include "tabix.hpp"
+
+ const short INNIE = 1;
+ const short OUTTIE = 2;
+--- a/src/task_fcdrate.cpp
++++ b/src/task_fcdrate.cpp
+@@ -9,7 +9,6 @@
+
+ #include "utils.h"
+ #include "histogram.h"
+-#include "tabix/tabix.hpp"
+
+ using namespace std;
+
+--- a/src/reapr.pl
++++ b/src/reapr.pl
+@@ -12,8 +12,8 @@
+ $this_script = File::Spec->rel2abs($this_script);
+ my ($scriptname, $scriptdir) = fileparse($this_script);
+ $scriptdir = File::Spec->rel2abs($scriptdir);
+-my $tabix = File::Spec->catfile($scriptdir, 'tabix/tabix');
+-my $bgzip = File::Spec->catfile($scriptdir, 'tabix/bgzip');
++my $tabix = 'tabix';
++my $bgzip = 'bgzip';
+ my $version = '1.0.18';
+
+ if ($#ARGV == -1) {
+--- a/src/task_preprocess.pl
++++ b/src/task_preprocess.pl
+@@ -52,9 +52,9 @@
+ my $ideal_fcd_file = File::Spec->catfile($sample_dir, 'ideal_fcd.txt');
+ my $lowess_prefix = File::Spec->catfile($sample_dir, 'gc_vs_cov.lowess');
+ my $r_script = File::Spec->catfile($sample_dir, 'gc_vs_cov.R');
+-my $tabix = File::Spec->catfile($scriptdir, 'tabix/tabix');
+-my $bgzip = File::Spec->catfile($scriptdir, 'tabix/bgzip');
+-my $samtools = File::Spec->catfile($scriptdir, 'samtools');
++my $tabix = 'tabix';
++my $bgzip = 'bgzip';
++my $samtools = 'samtools';
+
+ # make directory and soft links to required files
+ $fasta_in = File::Spec->rel2abs($fasta_in);
+--- a/src/task_perfectfrombam.pl
++++ b/src/task_perfectfrombam.pl
+@@ -57,12 +57,12 @@
+ my $min_perfect_map_qual = $ARGV[5];
+ my $min_align_score = $ARGV[6];
+ my $bam2perfect = File::Spec->catfile($scriptdir, 'bam2perfect');
+-my $bgzip = File::Spec->catfile($scriptdir, 'tabix/bgzip');
+-my $tabix = File::Spec->catfile($scriptdir, 'tabix/tabix');
++my $bgzip = 'bgzip';
++my $tabix = 'tabix';
+ my $ERROR_PREFIX = '[REAPR perfectfrombam]';
+ my $perfect_bam = "$out_prefix.tmp.perfect.bam";
+ my $repetitive_bam = "$out_prefix.tmp.repetitive.bam";
+-my $samtools = File::Spec->catfile($scriptdir, 'samtools');
++my $samtools = 'samtools';
+ my %seq_lengths;
+ my %used_seqs;
+ my $hist_file = "$out_prefix.hist";
+--- a/src/task_perfectmap.pl
++++ b/src/task_perfectmap.pl
+@@ -38,14 +38,14 @@
+ my $reads_2 = $ARGV[2];
+ my $fragsize = $ARGV[3];
+ my $preout = $ARGV[4];
+-my $findknownsnps = File::Spec->catfile($scriptdir, 'findknownsnps');
++my $findknownsnps = 'findknownsnps';
+ my $ERROR_PREFIX = '[REAPR perfect_map]';
+ my $raw_coverage_file = "$preout.tmp.cov.txt";
+ my $tmp_bin = "$preout.tmp.bin";
+ my $tmp_bin_single_match = "$tmp_bin\_single_match.fastq";
+-my $tabix = File::Spec->catfile($scriptdir, 'tabix/tabix');
+-my $bgzip = File::Spec->catfile($scriptdir, 'tabix/bgzip');
+-my $samtools = File::Spec->catfile($scriptdir, 'samtools');
++my $tabix = 'tabix';
++my $bgzip = 'bgzip';
++my $samtools = 'samtools';
+ my $all_bases_outfile = "$preout.perfect_cov.gz";
+ my $hist_outfile = "$preout.hist";
+ my @coverage = (0) x 101;
+--- a/src/task_plots.pl
++++ b/src/task_plots.pl
+@@ -50,9 +50,9 @@
+ }
+
+
+-my $tabix = File::Spec->catfile($scriptdir, 'tabix/tabix');
+-my $bgzip = File::Spec->catfile($scriptdir, 'tabix/bgzip');
+-my $samtools = File::Spec->catfile($scriptdir, 'samtools');
++my $tabix = 'tabix';
++my $bgzip = 'bgzip';
++my $samtools = 'samtools';
+ my @plot_list = ('frag_cov', 'frag_cov_cor', 'read_cov', 'read_ratio_f', 'read_ratio_r', 'clip', 'FCD_err');
+ my @file_list;
+ my $fa_out = "$outprefix.ref.fa";
+--- a/src/task_pipeline.pl
++++ b/src/task_pipeline.pl
+@@ -9,7 +9,7 @@
+
+ my ($scriptname, $scriptdir) = fileparse($0);
+ my $reapr_dir = abs_path(File::Spec->catfile($scriptdir, File::Spec->updir()));
+-my $reapr = File::Spec->catfile($reapr_dir, 'reapr');
++my $reapr = 'reapr';
+
+ my %options = (fcdcut => 0);
+
+--- a/src/task_seqrename.pl
++++ b/src/task_seqrename.pl
+@@ -7,7 +7,7 @@
+ use Getopt::Long;
+
+ my ($scriptname, $scriptdir) = fileparse($0);
+-my $samtools = File::Spec->catfile($scriptdir, 'samtools');
++my $samtools = 'samtools';
+ my %options;
+ my $usage = qq/<rename file> <in.bam> <out.bam>
+
+--- a/src/task_smaltmap.pl
++++ b/src/task_smaltmap.pl
+@@ -77,8 +77,8 @@
+ my $reads_2 = $ARGV[2];
+ my $final_bam = $ARGV[3];
+ my $ERROR_PREFIX = '[REAPR smaltmap]';
+-my $samtools = File::Spec->catfile($scriptdir, 'samtools');
+-my $smalt = File::Spec->catfile($scriptdir, 'smalt');
++my $samtools = 'samtools';
++my $smalt = 'smalt';
+ my $tmp_prefix = "$final_bam.tmp.$$.smaltmap";
+ my $smalt_index = "$tmp_prefix.smalt_index";
+ my $smalt_sample = "$tmp_prefix.smalt_sample";
diff --git a/sci-biology/reapr/reapr-1.0.18.ebuild b/sci-biology/reapr/reapr-1.0.18.ebuild
index 312219a..893af8b 100644
--- a/sci-biology/reapr/reapr-1.0.18.ebuild
+++ b/sci-biology/reapr/reapr-1.0.18.ebuild
@@ -2,7 +2,9 @@
# Distributed under the terms of the GNU General Public License v2
# $Id$
-EAPI=5
+EAPI=6
+
+inherit eutils
DESCRIPTION="Verify and correct genome assembly scaffolds using paired-end reads"
HOMEPAGE="http://www.sanger.ac.uk/science/tools/reapr"
@@ -11,7 +13,7 @@ SRC_URI="ftp://ftp.sanger.ac.uk/pub/resources/software/reapr/Reapr_${PV}.tar.gz
LICENSE="GPL-3"
SLOT="0"
-KEYWORDS="" # does not link against -lbamtools
+KEYWORDS=""
IUSE=""
# tested smalt versions 0.6.4 to 0.7.0.1 only
@@ -28,11 +30,15 @@ RDEPEND="${DEPEND}"
S="${WORKDIR}"/Reapr_"${PV}"
+# we use temporarily patches from https://anonscm.debian.org/cgit/debian-med/reapr.git/tree/debian/patches
+
src_prepare(){
+ default
+ for f in "${FILESDIR}"/*.patch; do epatch $f || die; done
sed -e 's#^CC = g++#CXX ?= g++#' -i src/Makefile || die
sed -e 's#$(CC)#$(CXX)#' -i src/Makefile || die
sed -e 's#-O3##' -i src/Makefile || die
- sed -e 's#^CFLAGS =#CXXFLAGS += -I../third_party/bamtools/src -L../third_party/bamtools/src#' -i src/Makefile || die
+ sed -e 's#^CFLAGS =#CXXFLAGS += -I../third_party/tabix -L../third_party/tabix -I../third_party/bamtools/src -L../third_party/bamtools/src#' -i src/Makefile || die
#sed -e 's#-lbamtools#../third_party/bamtools/src/libbamtools.so#' -i src/Makefile || die
sed -e 's#-ltabix#../third_party/tabix/libtabix.a#' -i src/Makefile || die
sed -e 's#CFLAGS#CXXFLAGS#' -i src/Makefile || die
^ permalink raw reply related [relevance 99%]
Results 1-1 of 1 | reverse | options above
-- pct% links below jump to the message on this page, permalinks otherwise --
2016-03-31 22:52 99% [gentoo-commits] proj/sci:master commit in: sci-biology/reapr/files/, sci-biology/reapr/ Martin Mokrejs
This is a public inbox, see mirroring instructions
for how to clone and mirror all data and code used for this inbox